ICAM5 antibody (C-Term)

Catalog No. ABIN453983

Product Details anti-ICAM5 Antibody

Target: ICAM5 (Intercellular Adhesion Molecule 5 (ICAM5))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ICAM5 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes ICAM5 precursor.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ESPAEGEVFAIQ, from the C Terminus of the protein sequence according to NP_003250.2.

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/128000. Western Blot: Preliminary experiments gave an approx 200-250 kDa band in lysates ofHuman Brain (Frontal Cortex and Hippocampus) and Mouse Brain after 0.05 μg/mLantibody staining. Please note that currently we cannot find an explanation in the literaturefor the band we observe given the calculated size of 97.0 kDa according to NP_003250.2. The 200-250 kDa band was successfully blocked by incubation with the immunizingpeptide. Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Brain (Cerebral Cortex)shows axon/dendrite staining leaving cell bodies relatively unstained.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for ICAM5

Target: ICAM5 (Intercellular Adhesion Molecule 5 (ICAM5))

Alternative Name: ICAM5 (ICAM5 Products)

Synonyms: TLCN antibody, TLN antibody, CD50 antibody, ICAM-3 antibody, Icam3 antibody, Tlcn antibody, ICAM5 antibody, icam5 antibody, tlcn antibody, tln antibody, intercellular adhesion molecule 5 antibody, intercellular adhesion molecule 5, telencephalin antibody, intercellular adhesion molecule 5 L homeolog antibody, ICAM5 antibody, Icam5 antibody, LOC100511183 antibody, icam5.L antibody

Background: ICAM5 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.Synonyms: ICAM-5, Intercellular Adhesion Molecule 5, TLCN, TLN, Telencephalin

Gene ID: 7087

NCBI Accession: NP_003250

UniProt: Q9UMF0

Pathways: Cell-Cell Junction Organization, ER-Nucleus Signaling, Maintenance of Protein Location