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Mouse anti-Human IgM Antibody (HRP)

ELISA, WB Monoclonal CH2 IgG1 HRP
Catalog No. ABIN457359
  • Target See all IgM products
    IgM
    Reactivity
    • 188
    • 130
    • 65
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    Human
    Host
    • 321
    • 188
    • 72
    • 15
    • 14
    • 5
    • 4
    • 1
    • 1
    Mouse
    Clonality
    • 496
    • 77
    Monoclonal
    Conjugate
    • 119
    • 76
    • 76
    • 55
    • 36
    • 32
    • 24
    • 15
    • 12
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    HRP
    Application
    • 332
    • 250
    • 238
    • 208
    • 143
    • 139
    • 64
    • 63
    • 47
    • 47
    • 46
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB)
    Specificity
    The antibody CH2 reacts with Fc fragment of human IgM.
    Cross-Reactivity (Details)
    Human
    Purification
    Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.
    Immunogen
    Purified human IgM.
    Clone
    CH2
    Isotype
    IgG1
  • Application Notes
    Western blotting: Recommended dilution: 1 μg/mL.
    Flow cytometry: Recommended dilution: 1-4 μg/mL, extracellular and intracellular staining.
    Comment

    The purified antibody is conjugated with Horseradish Peroxidase (HRP) of high specific activity and RZ=3.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01 % thimerosal
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
    Background
    Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.,immunoglobulin M
    Molecular Weight
    900 kDa
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