Mouse anti-Human IgM (Fc Region) Antibody (HRP)

Details for Product No. ABIN457359, Supplier: Log in to see
Antigen
Epitope
Fc Region
558
373
290
63
56
51
46
28
6
6
2
1
1
1
1
Reactivity
Human
1190
559
232
92
80
67
66
58
56
56
56
40
35
28
19
15
15
7
5
5
2
1
1
1
Host
Mouse
1262
664
511
143
38
11
10
9
4
3
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
HRP
356
343
271
157
99
74
67
53
25
25
23
22
22
19
18
16
16
16
16
15
14
14
13
12
9
7
7
5
5
5
4
4
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
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Immunogen Purified human IgM.
Clone CH2
Isotype IgG1
Specificity The antibody CH2 reacts with Fc fragment of human IgM.
Characteristics The purified antibody is conjugated with Horseradish Peroxidase (HRP) of high specific activity and RZ=3.
Background Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Molecular Weight 900 kDa
Research Area Secondary Antibodies
Application Notes The antibody is designed for Western blotting analysis. Suggested working concentration is 4 µg/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 0.01 % (w/v) thimerosal and 1 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Flow Cytometry (FACS) image for Mouse anti-Human IgM (Fc Region) Antibody (HRP) (ABIN457359) Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC. Cells...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).