CD28 (CD28) antibody

Antigen: CD28 (CD28)

Synonyms: Tp44, MGC138290

Clonality: Monoclonal (37-51)

Host: Hamster

Reactivity: Mouse (Murine)

Catalog no.: ABIN457400

Additional Information

Alternative name: CD28

Immunogen: Mouse T cell lymphoma EL-4 cells

Format: Low Endotoxin

Isotype: IgG  (Matching secondary antibodies)

Clone: 37-51

Description: CD28 is the critical T cell costimulatory receptor which provides to the cell the importantsecond activation signal by binding CD80 and CD86 that are expressed by antigenpresenting cells. Besides its costimulation role CD28 functions in preventing T cells fromanergic hyporesponsive state or from undergoing premature apoptotic cell death. In murineperipheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. Inthe thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, andon CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 onmature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on theimmature cells.

Specificity: The hamster monoclonal antibody 37.51 reacts with extracellular domain of mouse CD28costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature Tcells and NK cells.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from cell culture supernatant by protein-G affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized. Endotoxin level is less than 0.1 EU/ µ g of the protein, as determined by the LAL test.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles. This low endotoxin solution contains no preservative, handle under aseptic conditions.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis

Restrictions: For Research Use only