Intercellular Adhesion Molecule 1 (ICAM1) antibody

Antigen: Intercellular Adhesion Molecule 1 (ICAM1)

Synonyms: BB2, CD54, P3.58, Ly-47, Icam-1, MALA-2, MGC6195, ICAM-1, ICAM1, ICAM

Clonality: Monoclonal (YN1-1-7-4)

Host: Rat

Reactivity: Mouse (Murine)

Catalog no.: ABIN457408

Additional Information

Alternative name: CD54

Immunogen: Mouse NS-1 cells

Format: Low Endotoxin

Isotype: IgG2b  (Matching secondary antibodies)

Clone: YN1-1-7-4

Description: CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is atransmembrane molecule with 7 potential N-glycosylated sites, expressed on restingmonocytes and endothelial cells and can be upregulated on many other cells, e.g. withlymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also onkeratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion bybinding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction ofCD54 with LFA-1 enhances antigen-specific T-cell activation.

Specificity: The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type Itransmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, Blymphocytes, monocytes, macrophages, granulocytes and dendritic cells, the expression ofCD54 is upregulated by activation.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from cell culture supernatant by protein-G affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized. Endotoxin level is less than 0.1 EU/ µ g of the protein, as determined by the LAL test.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: CD Antigens, Surface Receptors of Immune Cells, Immunology, Inflammation, Infection

Restrictions: For Research Use only