CD44 antibody

Catalog No. ABIN457426

Product Details anti-CD44 Antibody

Target: CD44

Reactivity: Human, Mouse, Dog, Horse, Cat

Host: Rat

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: The rat monoclonal antibody IM7 reacts with an extracellular epitope of CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.), it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.

Cross-Reactivity (Details): Human, Mouse, Canine (Dog), Equine (Horse), Feline (Cat)

Purification: Purified by protein-G affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1

Clone: IM7

Isotype: IgG2b kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1 μg/mL, positive control: murine peripheral blood.
Immunohistochemistry (paraffin sections): Positive tissue: murine spleen.
Western blotting: Non-reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD44 antibody (ABIN457426)

Hegde, Singh, Nagarkatti, Nagarkatti: "CD44 mobilization in allogeneic dendritic cell-T cell immunological synapse plays a key role in T cell activation." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 134-42, (2008) (PubMed).

Larkin, Renukaradhya, Sriram, Du, Gervay-Hague, Brutkiewicz: "CD44 differentially activates mouse NK T cells and conventional T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 268-79, (2006) (PubMed).

van Royen, Voskuil, Hoefer, Jost, de Graaf, Hedwig, Andert, Wormhoudt, Hua, Hartmann, Bode, Buschmann, Schaper, van der Neut, Piek, Pals: "CD44 regulates arteriogenesis in mice and is differentially expressed in patients with poor and good collateralization." in: Circulation, Vol. 109, Issue 13, pp. 1647-52, (2004) (PubMed).

Katoh, Matsumoto, Kawakita, Tominaga, Kincade, Matsukura: "A role for CD44 in an antigen-induced murine model of pulmonary eosinophilia." in: The Journal of clinical investigation, Vol. 111, Issue 10, pp. 1563-70, (2003) (PubMed).

Legg, Lewis, Parsons, Ng, Isacke: "A novel PKC-regulated mechanism controls CD44 ezrin association and directional cell motility." in: Nature cell biology, Vol. 4, Issue 6, pp. 399-407, (2002) (PubMed).

McKallip, Do, Fisher, Robertson, Nagarkatti, Nagarkatti: "Role of CD44 in activation-induced cell death: CD44-deficient mice exhibit enhanced T cell response to conventional and superantigens." in: International immunology, Vol. 14, Issue 9, pp. 1015-26, (2002) (PubMed).

Xu, Manivannan, Liversidge, Sharp, Forrester, Crane: "Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier." in: Journal of leukocyte biology, Vol. 72, Issue 6, pp. 1133-41, (2002) (PubMed).

Si-Tahar, Sitaraman, Shibahara, Madara: "Negative regulation of epithelium-neutrophil interactions via activation of CD44." in: American journal of physiology. Cell physiology, Vol. 280, Issue 3, pp. C423-32, (2001) (PubMed).

Target Details for CD44

Target: CD44

Alternative Name: CD44 (CD44 Products)

Synonyms: CDW44 antibody, CSPG8 antibody, ECMR-III antibody, HCELL antibody, HUTCH-I antibody, IN antibody, LHR antibody, MC56 antibody, MDU2 antibody, MDU3 antibody, MIC4 antibody, Pgp1 antibody, AU023126 antibody, AW121933 antibody, AW146109 antibody, HERMES antibody, Ly-24 antibody, Pgp-1 antibody, CD44A antibody, METAA antibody, RHAMM antibody, XCD44 antibody, cd44 antibody, cdw44 antibody, cspg8 antibody, ecmr-iii antibody, hcell antibody, lhr antibody, mc56 antibody, mdu2 antibody, mdu3 antibody, mic4 antibody, mutch-i antibody, pgp1 antibody, CD44 molecule (Indian blood group) antibody, syndecan 4 antibody, CD44 antigen antibody, CD44 molecule (Indian blood group) S homeolog antibody, CD44 antibody, SDC4 antibody, Cd44 antibody, cd44.S antibody

Background: CD44 Molecule,CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.,PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4

Gene ID: 12505

UniProt: P15379

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling