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Intercellular Adhesion Molecule 1 (ICAM1) antibody

Antigen

Intercellular Adhesion Molecule 1 (ICAM1)
Synonyms BB2, CD54, P3.58, Ly-47, Icam-1, MALA-2, MGC6195, ICAM-1, ICAM1, ICAM
Clonality Monoclonal (YN1-1-7-4)
Host
Reactivity
Alternatives

Mouse (Murine)
Application
Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunocytochemistry (ICC)
Catalog no. ABIN457439
Quantity 0.1 mg  (1 mg/ml)  (Variants)
Price 195.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name CD54
Immunogen Mouse NS-1 cells
Format Purified
Isotype IgG2b  (Matching secondary antibodies)
Clone YN1-1-7-4
Description CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is atransmembrane molecule with 7 potential N-glycosylated sites, expressed on restingmonocytes and endothelial cells and can be upregulated on many other cells, e.g. withlymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also onkeratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion bybinding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction ofCD54 with LFA-1 enhances antigen-specific T-cell activation.
Specificity The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type Itransmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, Blymphocytes, monocytes, macrophages, granulocytes and dendritic cells, the expression ofCD54 is upregulated by activation.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Flow Cytometry: Recommended dilution: 1 µg/ml. Application note: non-reducing conditionsImmunohistochemistry (frozen sections). Application note: acetone fixation. Functional Application: Blocking
Concentration 1 mg/ml
Purity > 95% (by SDS-PAGE)
Purification Purified from hybridoma culture supernatant by protein-G affinity chromatography.
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Inflammation, Infection
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Intercellular Adhesion Molecule 1 (ICAM1)", type "Antibodies"
Hosts Mouse (190), Rabbit (182), Rat (52), Hamster (4), Sheep (1)
Reactivities Human (339), Mouse (Murine) (140), Rat (Rattus) (97), Cow (Bovine) (56), Pig (Porcine) (44), Rabbit (43), Dog (Canine) (38), Sheep (Ovine) (18), Horse (Equine) (16), Chicken (4), Guinea Pig (2), Fish (1)
Applications Flow Cytometry (FACS) (327), Immunofluorescence (IF) (135), Western Blotting (WB) (125), Immunoprecipitation (IP) (80), Immunohistochemistry (Frozen Sections) (IHC (fro)) (75), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (62), ELISA (61), Immunohistochemistry (IHC) (20), Functional Studies (Func) (18), Enzyme Immunoassay (EIA) (14), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Blocking Antibody (Inhibition) (10), Immunocytochemistry (ICC) (10), Immunoelectron Microscopy (IEM) (7), Blocking Antibody (Inhibition) (6), Immunohistochemistry (Fixed) (IHC (fx)) (5), ELISA (Detection) (2)
Conjugates Biotin (42), FITC (38), PE (29), Alexa Fluor 488 (8), Alexa Fluor 350 (7), Alexa Fluor 555 (7), Alexa Fluor 647 (7), Cy3 (7), Cy5 (7), Cy5.5 (7), Cy7 (7), Gold (7), HRP (7), PE,Cy3 (7), PE,Cy5 (7), PE,Cy5.5 (7), PE,Cy7 (7), RBITC (7), RPE (7), APC (6), Alexa Flour 488 (6), Alexa Fluor 700 (1), Pacific Blue (1), PerCP (1)
Epitopes pTyr512 (9), C-Term (2), AA 253-267 (1), Domain D1 (1)