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Mouse anti-Human IgE Antibody (Biotin)

ELISA, WB, FACS, Func Monoclonal BSW17-NBS01 IgG1 kappa Biotin
Catalog No. ABIN457503
  • Target See all IgE products
    IgE
    Reactivity
    • 48
    • 30
    • 10
    • 10
    • 5
    • 4
    • 2
    • 1
    Human
    Host
    • 58
    • 18
    • 10
    • 10
    • 4
    • 1
    Mouse
    Clonality
    • 66
    • 28
    Monoclonal
    Conjugate
    • 26
    • 17
    • 16
    • 15
    • 8
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 55
    • 39
    • 30
    • 20
    • 20
    • 15
    • 12
    • 10
    • 10
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
    Specificity
    Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
    Sensitivity
    Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)
    Characteristics
    IGHE, chromosome 14q32.33
    Purification
    Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck
    Purity
    > 85 %, tested by analytical Gel Permeation Chromatography
    Sterility
    Sterile filtered
    Immunogen
    purified human myeloma IgE
    Clone
    BSW17-NBS01
    Isotype
    IgG1 kappa
  • Application Notes
    Sterile filtered solution, does not contain Na-azide. Recommended for functional cellular assays (mast cell / basophil activation).
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS, pH=7.2, sterile filtered, contains no NaN3
    Preservative
    Azide free
    Handling Advice
    Aliquot to Avoid repeated freezing and thawing.
    Storage
    4 °C
  • Target
    IgE
    Abstract
    IgE Products
    Background
    The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
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