Rabbit anti-Goat IgA, IgG, IgM (Heavy & Light Chain) Antibody
ID, IEP, RID
Polyclonal
IgG
unconjugated
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- Target See all IgA, IgG, IgM products
- IgA, IgG, IgM
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Goat
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Host
- Rabbit
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Clonality
- Polyclonal
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Application
- Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
- Specificity
- The reactivity of the antiserum is directed to the Fc and Fab subunits of the major immuno-globulins.
- Characteristics
- Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal goat serum in agar block titration.
- Purification
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Delipidated, heat inactivated, lyophilized stable whole serum
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity with other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. - Immunogen
- Purified normal Ig fractions isolated from pooled goat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
- Isotype
- IgG
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- Application Notes
- In immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal goat serum.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- It is reconstituted by adding 1 mL sterile distilled water.
- Buffer
- No foreign proteins added.
- Preservative
- Without preservative
- Handling Advice
- Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Target
- IgA, IgG, IgM
- Alternative Name
- IgG + IgA + IgM (IgA, IgG, IgM Products)
- Target Type
- Antibody
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