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Mouse anti-Human IgG (Fc Region) Antibody

ELISA, WB, IF Monoclonal NI 335 and NI 343 IgG1 kappa unconjugated

Catalog No. ABIN457826

Datasheet as PDF

Target See all IgG products

IgG
Binding Specificity
2333

499

411

188

129

122

92

29

28

28

15

9

6

6

6

6

5

2

2

1

1

1

1
Fc Region
Reactivity
692

672

558

451

366

239

198

147

137

131

122

98

95

95

92

62

46

33

27

17

12

10

8

5

5

4

3

3

3

3

3

3

3

3

2

2

2

1

1

1

1
Human
Host
1716

1474

537

203

118

103

33

31

21

17

15

11

11

1
Mouse
Clonality
3347

164

1
Monoclonal
Application
1837

1789

1785

1499

960

934

913

515

234

115

115

111

100

77

59

50

50

49

32

27

25

21

17

16

11

8

5

4

4

3

2

2

2

2

2

1

1

1

1

1
ELISA, Western Blotting (WB), Immunofluorescence (IF)

Specificity

The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. It reacts with the 4 subclasses of human IgG, as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. Monoclonal mouse antibody to human IgG, isotype specific

Purification

Purified

Immunogen

Highly purified monoclonal IgG isolated from human serum

Clone

NI 335 and NI 343

Isotype

IgG1 kappa
Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP

Goat anti-Human IgG (Heavy & Light Chain) antibody (Biotin) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG Biotin

Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP

Goat anti-Human IgG (F(ab')2 Region) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment

Goat anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

Mouse anti-Human IgG (Heavy & Light Chain) antibody
ELISA, WB Monoclonal 4D2D9G8-4A10 IgG1 unconjugated

Mouse anti-Human IgG (Fc Region) antibody (HRP)
ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP

Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated

Rabbit anti-Human IgG (Fc Region) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP

Goat anti-Human IgG (Fc Region) antibody (HRP)
ELISA, WB Polyclonal HRP F(ab')2 fragment

Application Notes

To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG, and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration

0.4 mg/mL

Buffer

Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative

Without preservative

Handling Advice

Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage

4 °C/-20 °C

Storage Comment

The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
Target

IgG

Abstract

IgG Products

Target Type

Antibody