Mouse anti-Human IgG1 (Fc Region) Antibody

Catalog No. ABIN457827

Product Details

Target: IgG1

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc part of the IgG1 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. Monoclonal mouse antibody to human IgG1, subclass specific

Purification: Purified

Immunogen: Highly purified monoclonal IgG1 isolated from human serum

Clone: NI 132 (HP 6186)

Isotype: IgG1 kappa

Application Details

Application Notes: To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG1, and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage,this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 24 °C.

Target Details

Target: IgG1

Abstract: IgG1 Products

Target Type: Antibody