He antiserum does not react with any other component of the human milk or plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Characteristics
Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human lactoferrin
Purification
Purified
Immunogen
Highly purified lactoferrin isolated from human milk.
To identify the presence of lactoferrin in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 upwards. These data should be interpreted as general recommendations only.
Restrictions
For Research Use only
Format
Lyophilized
Concentration
IgG concentration is 0.4 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) approximately 1.0. No foreign proteins added.
Buffer
Purified monoclonal mouse IgG1 kappa conjugated with fluorescein isothiocyanate, lyophilized from a solution in phosphate buffered saline (pH7.2).
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized product by addin g 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.