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Goat anti-Human IgM (Fc Region) Antibody (FITC) - Preadsorbed

IF Polyclonal IgG FITC F(ab')2 fragment
Catalog No. ABIN458075
  • Target See all IgM products
    IgM
    Fragment
    F(ab')2 fragment
    Binding Specificity
    • 141
    • 118
    • 86
    • 41
    • 17
    • 8
    • 7
    • 1
    • 1
    Fc Region
    Reactivity
    • 188
    • 130
    • 65
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    Human
    Host
    • 320
    • 188
    • 73
    • 15
    • 14
    • 5
    • 4
    • 1
    • 1
    Goat
    Clonality
    • 495
    • 78
    Polyclonal
    Conjugate
    • 119
    • 77
    • 75
    • 55
    • 36
    • 32
    • 24
    • 15
    • 12
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 333
    • 250
    • 238
    • 209
    • 142
    • 139
    • 64
    • 63
    • 47
    • 47
    • 46
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF)
    Specificity
    The antiserum is reacting with to the Fc subunit of IgM.
    Characteristics
    Fluorescein isothiocyanate-conjugated F(ab')2 fragments IgG antibodies of polyclonal goat antiserum to human IgM, Fc specific
    Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.6.
    Purification
    Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
    Immunogen
    Purified IgM prepared from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Application Notes
    Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgM. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. This immunoconjugate is not pre-diluted.
    The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.
    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
    Concentration
    10 mg/mL
    Buffer
    PBS, pH 7.2
    Preservative
    Without preservative
    Handling Advice
    Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
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