Goat anti-Human IgE (Fc Region) Antibody

Catalog No. ABIN458225

Product Details

Target: IgE

Binding Specificity: Fc Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

Specificity: Specificity Reference Reagent. Purified IgG fraction of polyclonal Goat antiSerum to Human IgE, Fc specific.

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail because of the low level of IgE in most species.

Characteristics: The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various erum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with human IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in human serum. As unlabelled secondary antibody for indirect detection of IgE in human cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in human serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.

Purification: Purified

Immunogen: Purified homogenous IgE isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: IgE

Abstract: IgE Products