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Goat anti-Mouse IgA, IgG, IgM (Fc Region) Antibody

ICC, IEP Polyclonal IgG unconjugated
Catalog No. ABIN458344
  • Target See all IgA, IgG, IgM products
    IgA, IgG, IgM
    Binding Specificity
    • 75
    • 58
    • 30
    • 16
    • 9
    Fc Region
    Reactivity
    • 72
    • 46
    • 36
    • 28
    • 12
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    Mouse
    Host
    • 160
    • 55
    • 4
    • 3
    • 1
    Goat
    Clonality
    • 218
    • 1
    Polyclonal
    Application
    • 117
    • 112
    • 77
    • 73
    • 68
    • 55
    • 26
    • 24
    • 23
    • 21
    • 15
    • 15
    • 13
    • 12
    • 12
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunocytochemistry (ICC), Immunoelectrophoresis (IEP)
    Specificity
    The reactivity of the antiserum is directed to the Fc subunits of the major immunoglobulins.
    Characteristics
    Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. Antibody titre: Precipitin titre not less than 1:128 when tested against normal mouse serum in agar block titration.
    Purification
    Delipidated, heat inactivated, lyophilized stable whole serum
    Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
    Immunogen
    Purified polyclonal IgG and homogenous IgA and IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Application Notes
    In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal mouse serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is reconstituted by adding 1 mL sterile distilled water.
    Buffer
    No foreign proteins added.
    Preservative
    Without preservative
    Handling Advice
    Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Target
    IgA, IgG, IgM
    Alternative Name
    IgG + IgA + IgM (IgA, IgG, IgM Products)
    Target Type
    Antibody
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