Rabbit anti-Sheep IgM (Fc Region) Antibody
ELISA, WB, IF, DB
Polyclonal
unconjugated
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- Target See all IgM products
- IgM
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Binding Specificity
- Fc Region
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Reactivity
- Sheep
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Host
- Rabbit
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Clonality
- Polyclonal
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Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)
- Specificity
- IgG fraction of polyclonal Rabbit antiSerum to Sheep IgM, Fc specific.
- Cross-Reactivity (Details)
- Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however a strong reaction with Goat IgM has been observed.
- Characteristics
- The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in sheep serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
- Purification
- Purified
- Immunogen
- Purified normal IgM isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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- Application Notes
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below - 20 °C.Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. IgG protein concentration 10 mg/mL. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below - 20 °C.
- Buffer
- Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. IgG protein concentration 10 mg/mL. No foreign proteins added.
- Preservative
- Without preservative
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
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