E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial))

Details for Product anti-CDH1 Antibody No. ABIN459320, Supplier: Log in to see
Antigen
  • Arc-1
  • CD324
  • CDHE
  • ECAD
  • LCAM
  • UVO
  • E-cadherin
  • cdh1
  • si:dz180o5.2
  • dZ71B9.1
  • si:dz180o5.1
  • uvo
  • lcam
  • E-Cad
  • XBcad
  • l-cam
  • xcdh1
  • cdhc-A
  • xb-cad
  • XTCAD-1
  • c-cadherin
  • cadherin-1
  • uvomorulin
  • XB-cadherin
  • CDH1
  • CDH16
  • HPT-1
  • HPT1
  • arc-1
  • cd324
  • cdhe
  • ecad
  • cad17
  • hpt1
  • cdh16
  • hpt-1
  • LI-cadherin
  • cadherin-17
  • b-cadherin
  • cdh1-a
  • cdhp
  • hjmd
  • pcad
  • AA960649
  • E-cad
  • Ecad
  • L-CAM
  • Um
  • Arc1
  • cadherin 1, type 1, E-cadherin (epithelial)
  • si:busm1-180o5.2
  • cadherin 1, epithelial
  • cadherin 1
  • si:busm1-180o5.1
  • cadherin 17, LI cadherin (liver-intestine)
  • Cadherin-17
  • cadherin 3, type 1, P-cadherin (placental)
  • E-cadherin-like
  • Aerobic running capacity QTL 1
  • CDH1
  • si:busm1-180o5.2
  • cdh1
  • Cdh1
  • si:busm1-180o5.1
  • CDH17
  • cad17
  • cdh17
  • cdh3-a
  • LOC100356548
  • Arunc1
Alternatives
anti-Human E-cadherin antibody for Biochemical Assay
Reactivity
Human
789
238
146
40
26
16
9
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8
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3
1
1
1
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1
1
1
1
1
Host
Mouse
531
267
37
22
2
Clonality (Clone)
Monoclonal ()
Conjugate
This E-cadherin antibody is un-conjugated
42
38
33
23
20
20
16
15
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15
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12
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12
12
11
11
11
9
8
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3
3
3
3
3
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2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
623
351
269
200
173
173
158
88
87
38
14
14
13
10
10
7
6
6
4
2
2
2
2
2
1
1
1
1
Options
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Immunogen 5H9 is a mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with affinity purified 80 kD extracellular fragments of E-cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells.
Clone 5H9
Isotype IgG1
Specificity 5H9 recognizes both the 120 kD E-cadherin and its 80 kD trypsin-resistant extracellular part.
Purification Affinity purified
Alternative Name E-Cadherin/Cadherin-1 (CDH1 Antibody Abstract)
Background Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin E7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.
Pathways Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
Application Notes 5H9 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin embedded tissues. For frozen tissues use a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For paraffin embedded tissues use a pretreatment step of 15 minutes incubation in TRIS-EDTA buffer pH 9 in a microwave. Recommended dilution Optimal antibody dilution should be determined by titration, recommended range is 1:50 – 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:500 for immunoblotting applications.
Restrictions For Research Use only
Format Liquid
Concentration 1mg/ml
Buffer Each vial contains 100 ml of 1 mg/ml purified antibody in PBS containing 0.09% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
 image for anti-E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (ABIN459320) anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
Product cited in: Schwechheimer, Zhou, Birchmeier: "E-Cadherin in human brain tumours: loss of immunoreactivity in malignant meningiomas." in: Virchows Archiv : an international journal of pathology, Vol. 432, Issue 2, pp. 163-7, 1998 (PubMed).

Gabbert, Mueller, Schneiders, Meier, Moll, Birchmeier, Hommel: "Prognostic value of E-cadherin expression in 413 gastric carcinomas." in: International journal of cancer. Journal international du cancer, Vol. 69, Issue 3, pp. 184-9, 1996 (PubMed).

Moll, Mitze, Frixen, Birchmeier: "Differential loss of E-cadherin expression in infiltrating ductal and lobular breast carcinomas." in: The American journal of pathology, Vol. 143, Issue 6, pp. 1731-42, 1994 (PubMed).

Frixen, Behrens, Sachs, Eberle, Voss, Warda, Löchner, Birchmeier: "E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells." in: The Journal of cell biology, Vol. 113, Issue 1, pp. 173-85, 1991 (PubMed).