You are viewing an incomplete version of our website. Please click to reload the website as full version.

E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial))

Details for Product anti-CDH1 Antibody No. ABIN459321, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

E-Cad, E-cadherin, XB-cadherin, XBcad, XTCAD-1, b-cadherin, cadherin-1, cdh1-a, cdhc-A, cdhp, hjmd, l-cam, lcam, pcad, uvo, uvomorulin, xb-cad, xcdh1, Arc1, AA960649, E-cad, Ecad, L-CAM, UVO, Um, Arc- ... show more
E-Cad, E-cadherin, XB-cadherin, XBcad, XTCAD-1, b-cadherin, cadherin-1, cdh1-a, cdhc-A, cdhp, hjmd, l-cam, lcam, pcad, uvo, uvomorulin, xb-cad, xcdh1, Arc1, AA960649, E-cad, Ecad, L-CAM, UVO, Um, Arc-1, CD324, CDHE, ECAD, LCAM, arc-1, cd324, cdhe, ecad, cdh1, si:dz180o5.2 show less
(439), (130), (93), (22), (20), (11), (6), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(260), (198), (19), (17), (2), (1)
Clonality (Clone)
Monoclonal ()
This E-cadherin antibody is un-conjugated
(30), (24), (16), (14), (10), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(320), (144), (102), (99), (98), (66), (52), (48), (43), (32), (13), (9), (6), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 1 mL
Shipping to United States ( )
Availability Will be delivered in 5 to 7 Business Days
Immunogen 5H9 is a mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with affinity purified 80 kD extracellular fragments of E-cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells.
Clone 5H9
Isotype IgG1
Specificity 5H9 recognizes both the 120 kD E-cadherin and its 80 kD trypsin-resistant extracellular part.
Purification Culture Supernatant
Alternative Name E-Cadherin/Cadherin-1 (CDH1 Antibody Abstract)
Background Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin E7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.
Application Notes 5H9 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin embedded tissues. For frozen tissues use a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For paraffin embedded tissues use a pretreatment step of 15 minutes incubation in TRIS-EDTA buffer pH 8-9 in a microwave. Optimal antibody dilution should be determined by titration.
Restrictions For Research Use only
Format Liquid
Buffer Each vial contains 1 ml culture supernatant containing 0.09% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
 image for anti-E-cadherin antibody (Cadherin 1, Type 1, E-Cadherin (Epithelial)) (ABIN459321) anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
Product cited in: Schwechheimer, Zhou, Birchmeier: "E-Cadherin in human brain tumours: loss of immunoreactivity in malignant meningiomas." in: Virchows Archiv : an international journal of pathology, Vol. 432, Issue 2, pp. 163-7, 1998 (PubMed).

Gabbert, Mueller, Schneiders et al.: "Prognostic value of E-cadherin expression in 413 gastric carcinomas." in: International journal of cancer. Journal international du cancer, Vol. 69, Issue 3, pp. 184-9, 1996 (PubMed).

Moll, Mitze, Frixen et al.: "Differential loss of E-cadherin expression in infiltrating ductal and lobular breast carcinomas." in: The American journal of pathology, Vol. 143, Issue 6, pp. 1731-42, 1994 (PubMed).

Frixen, Behrens, Sachs et al.: "E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells." in: The Journal of cell biology, Vol. 113, Issue 1, pp. 173-85, 1991 (PubMed).

Catalog No. ABIN459321
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)