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Details for Product No. ABIN459385

Amyloid beta (Pyroglutamated/pGlu) Peptide antibody

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Antigen
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Application
»Alternatives Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN459385
Quantity 100 µL
Price
357.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen (3-8) N-terminal pyroglutamated synthetic amyloid beta peptide conjugated with a carrier protein.
Clone 2G6
Isotype IgG1
Specificity Based on amino acid sequence homology, reactivity with most vertebrates including dog, pig, and chicken is expected.
Characteristics Mouse monoclonal antibody against pyroglutamated amyloid beta (pGlu amyloid beta antibody)
Background Aβ pE3 (N-terminally truncated Aβ starting with pyroglutamate) represents a dominant fraction of Aβ peptides in senile plaques of AD brains. Aβ pE3 has higher aggregation propensity and shows increased toxicity compared to full-length Aβ. Intraneuronal accumulation of Aβ pE3 peptides induces severe neuron loss and an associated neurological phenotype. In APP/PS1KI mice, a continuous increase in Aβ pE3 plaque load with increasing age has been reported. It has been suggested that the peptides starting with position 1 of Aβ are N-truncated as disease progresses and that Aβ pE3 positive plaques are resistant to degradation likely due to their high stability and propensity to aggregate (1-5) .
Research Area Alzheimer's Disease
Application Notes IHC, ELISA, Western blotting. Immunohistochemistry: Tissue sections: (alcohol-fixed, paraffin embedded, frozen). Recommended antibody dilution - 1:100 -1:500. Tissue pretreatment with citrate buffer pH 6.0 (microwave boiling for 1-2 min), followed by a 3 minute incubation with 88% formic acid at RT. Western blotting: 1:1000-1:2000 ELISA : 1 : 2000 – 1 : 4000 Optimal dilutions for specific application should be determined by investigator. Appropriate concentration will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, length of incubation, etc.: Research Use Only.
Restrictions For Research Use only
Format Liquid
Concentration 2.5 mg/mL
Storage -20 °C
Tekirian, Saido, Markesbery et al.: "N-terminal heterogeneity of parenchymal and cerebrovascular Abeta deposits." in: Journal of neuropathology and experimental neurology, Vol. 57, Issue 1, pp. 76-94, 1998 (PubMed).

Russo, Violani, Salis et al.: "Pyroglutamate-modified amyloid beta-peptides--AbetaN3(pE)--strongly affect cultured neuron and astrocyte survival." in: Journal of neurochemistry, Vol. 82, Issue 6, pp. 1480-9, 2002 (PubMed).

Revesz, Holton, Lashley et al.: "Genetics and molecular pathogenesis of sporadic and hereditary cerebral amyloid angiopathies." in: Acta neuropathologica, Vol. 118, Issue 1, pp. 115-30, 2009 (PubMed).

Wirths, Breyhan, Cynis et al.: "Intraneuronal pyroglutamate-Abeta 3-42 triggers neurodegeneration and lethal neurological deficits in a transgenic mouse model." in: Acta neuropathologica, Vol. 118, Issue 4, pp. 487-96, 2009 (PubMed).

Wirths O, Bethge T, Marcello A et al.: "Pyroglutamate Abeta pathology in APP/PS1KI mice, sporadic and familial Alzheimer's disease cases" in: J Neural Transm, Vol. 57, Issue 1, pp. 85-96, 2010 (PubMed).

Alternatives for antigen "Amyloid beta (Pyroglutamated/pGlu) Peptide", type "Antibodies"
Hosts (1)
Reactivities (1), (1)
Applications (1), (1), (1)
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