Amyloid beta (Pyroglutamated/pGlu) Peptide antibody

Details for Product No. ABIN459385, Supplier: Log in to see
Human, Mouse (Murine)
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen (3-8) N-terminal pyroglutamated synthetic amyloid beta peptide conjugated with a carrier protein.
Clone 2G6
Isotype IgG1
Specificity Based on amino acid sequence homology, reactivity with most vertebrates including dog, pig, and chicken is expected.
Characteristics Mouse monoclonal antibody against pyroglutamated amyloid beta (pGlu amyloid beta antibody)
Background Aβ pE3 (N-terminally truncated Aβ starting with pyroglutamate) represents a dominant fraction of Aβ peptides in senile plaques of AD brains. Aβ pE3 has higher aggregation propensity and shows increased toxicity compared to full-length Aβ. Intraneuronal accumulation of Aβ pE3 peptides induces severe neuron loss and an associated neurological phenotype. In APP/PS1KI mice, a continuous increase in Aβ pE3 plaque load with increasing age has been reported. It has been suggested that the peptides starting with position 1 of Aβ are N-truncated as disease progresses and that Aβ pE3 positive plaques are resistant to degradation likely due to their high stability and propensity to aggregate (1-5) .
Research Area Alzheimer's Disease
Application Notes IHC, ELISA, Western blotting. Immunohistochemistry: Tissue sections: (alcohol-fixed, paraffin embedded, frozen). Recommended antibody dilution - 1:100 -1:500. Tissue pretreatment with citrate buffer pH 6.0 (microwave boiling for 1-2 min), followed by a 3 minute incubation with 88% formic acid at RT. Western blotting: 1:1000-1:2000 ELISA : 1 : 2000 – 1 : 4000 Optimal dilutions for specific application should be determined by investigator. Appropriate concentration will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, length of incubation, etc.: Research Use Only.
Restrictions For Research Use only
Format Liquid
Concentration 2.5 mg/mL
Storage -20 °C
Supplier Images
 image for anti-Amyloid beta (Pyroglutamated/pGlu) Peptide antibody (ABIN459385) Immunolabelling of senile plaques in cortex from 17 month-old triple-transgenic AD mo...
Western Blotting (WB) image for anti-Amyloid beta (Pyroglutamated/pGlu) Peptide antibody (ABIN459385) Lane 1 – 1 μg of synthetic pGlu 3-42 Aβ peptide per lane, Lane 2 – 5 μg of synthetic ...
Background publications Wirths O, Bethge T, Marcello A, Harmeier A, Jawhar S, Lucassen PJ, Multhaup G, Brody DL, Esparza T, Ingelsson M, Kalimo H, Lannfelt L, Bayer TA.: "Pyroglutamate Abeta pathology in APP/PS1KI mice, sporadic and familial Alzheimer's disease cases" in: J Neural Transm, Vol. 57, Issue 1, pp. 85-96, 2010 (PubMed).

Revesz, Holton, Lashley, Plant, Frangione, Rostagno, Ghiso: "Genetics and molecular pathogenesis of sporadic and hereditary cerebral amyloid angiopathies." in: Acta neuropathologica, Vol. 118, Issue 1, pp. 115-30, 2009 (PubMed).

Wirths, Breyhan, Cynis, Schilling, Demuth, Bayer: "Intraneuronal pyroglutamate-Abeta 3-42 triggers neurodegeneration and lethal neurological deficits in a transgenic mouse model." in: Acta neuropathologica, Vol. 118, Issue 4, pp. 487-96, 2009 (PubMed).

Russo, Violani, Salis, Venezia, Dolcini, Damonte, Benatti, DArrigo, Patrone, Carlo, Schettini: "Pyroglutamate-modified amyloid beta-peptides--AbetaN3(pE)--strongly affect cultured neuron and astrocyte survival." in: Journal of neurochemistry, Vol. 82, Issue 6, pp. 1480-9, 2002 (PubMed).

Tekirian, Saido, Markesbery, Russell, Wekstein, Patel, Geddes: "N-terminal heterogeneity of parenchymal and cerebrovascular Abeta deposits." in: Journal of neuropathology and experimental neurology, Vol. 57, Issue 1, pp. 76-94, 1998 (PubMed).