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Actin, beta (ACTB) antibody
| Antigen | Actin, beta (ACTB) |
| Synonyms |
PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A19 ...
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| Clonality | Monoclonal |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human, Rat (Rattus) |
| Application |
Alternatives Western Blotting (WB)
|
| Catalog no. | ABIN459390 |
| Quantity | 100µg (1 mg/ml) (Variants) |
| Price | 743.71 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Alternative name | beta-Actin |
| Format | Liquid |
| Description | All eukaryotic cells express actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. -actin expression is limited to various types of muscle, whereas – and -actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates ctin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia |
| Specificity | Beta -Actin antibody detects endogenous levels of beta -Actin protein. |
| Molecular Weight | 45 kDa |
| Synonyms | PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1 |
Application Details
| Application Notes | WB:1:2000- 1:5000 |
| Concentration | 1 mg/ml |
| Purity | > 95% (by SDS-PAGE). |
| Purification | The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen. |
| Buffer | 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "Actin, beta (ACTB)", type "Antibodies"
| Hosts | Rabbit (35), Mouse (31), Chicken (2) |
| Reactivities | Human (56), Rat (Rattus) (25), Mouse (Murine) (24), Chicken (11), Rabbit (8), Cow (Bovine) (7), Pig (Porcine) (6), Sheep (Ovine) (5), Cat (Feline) (3), Dog (Canine) (3), Guinea Pig (3), Hamster (3), Monkey (3), Carp (2), Fish (2), Fruit Fly (Drosophila melanogaster) (2), Goat (2), Primate (1) |
| Applications | Western Blotting (WB) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (20), Immunofluorescence (IF) (17), ELISA (15), Enzyme Immunoassay (EIA) (14), Immunohistochemistry (IHC) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunoprecipitation (IP) (5), Dot Blot (Dot) (4), Immunocytochemistry (ICC) (4), Flow Cytometry (FACS) (2), ELISA (Detection) (1) |
| Conjugates | HRP (2), Biotin (1) |
| Epitopes | N-Term (7), C-Term (1) |
