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Antibodies
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anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
Mitogen-Activated Protein Kinase 3 (MAPK3) antibody
| Antigen |
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| Synonyms |
ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180, zERK1, fi06b09, wu:fi06b09, Tb08.10J17.940 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (139), Rat (Rattus) (64), Mouse (Murine) (63), Cow (Bovine) (10), Chicken (7), Xenopus laevis (7), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Guinea Pig (2), Dog (Canine) (1), Primate (1), Rabbit (1), Zebrafish (Danio rerio) (1)
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB) (132), ELISA (34), Immunofluorescence (IF) (34), Enzyme Immunoassay (EIA) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (23), Immunoprecipitation (IP) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Dot Blot (Dot) (3), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
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| Catalog no. |
ABIN459502 |
| Quantity |
100µg (1 mg/ml) (Variants) |
| Price |
743.71 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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ERK1/2
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Format
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Liquid
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Description
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The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
Synonyms: MAPK1/2, Extracellular signaling related kinase 1/2
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Specificity
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ERK1/2 (L352) pAb detects endogenous levels of ERK1/2 protein.
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Molecular Weight
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42,44 kDa
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Application Notes
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WB: 1:500-1:1000 IHC: 1:50-1:200
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE).
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Buffer
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1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
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Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "Mitogen-Activated Protein Kinase 3 (MAPK3)", type "Antibodies"
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Hosts
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Rabbit (113), Mouse (30), Goat (6), Chicken (1), Sheep (1)
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Reactivities
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Human (139), Rat (Rattus) (64), Mouse (Murine) (63), Cow (Bovine) (10), Chicken (7), Xenopus laevis (7), Sheep (Ovine) (6), Fruit Fly (Drosophila melanogaster) (5), Guinea Pig (2), Dog (Canine) (1), Primate (1), Rabbit (1), Zebrafish (Danio rerio) (1)
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Applications
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Western Blotting (WB) (132), ELISA (34), Immunofluorescence (IF) (34), Enzyme Immunoassay (EIA) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (23), Immunoprecipitation (IP) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Dot Blot (Dot) (3), Immunocytochemistry (ICC) (2), ELISA (Detection) (1)
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Conjugates
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Biotin (1), HRP (1)
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Epitopes
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pTyr204 (8), N-Term (7), pThr202 (7), pThr202/pTyr204 (5), C-Term (4), pThr202,pTyr204 (2), pThr202/Tyr204 (2), D192 (1), T202 (1), Y204 (1), pT-E-pY Motif (1), pT202 (1), pY204 (1)
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