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Antibodies
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anti-Platelet-Derived Growth Factor beta Polypeptide (Simian Sarcoma Viral (V-Sis)...
Platelet-Derived Growth Factor beta Polypeptide (Simian Sarcoma Viral (V-Sis) Oncogene Homolog) (PDGFB) antibody
| Antigen |
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| Synonyms |
PDGFB, sis, c-sis, pdgf2, MGC83524, si:ch211-79m20.1, si:dkey-261d11.1 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (9), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (4), Functional Studies (Func) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN459678 |
| Quantity |
100µg (1 mg/ml) |
| Price |
743.71 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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PDGF-B
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Format
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Liquid
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Description
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PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB and PDGF BB. The three isoforms bind with different affinities to two receptor types, alpha and beta, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers, the A subunit of PDGF binds only to alpha receptors with high affinity, whereas the B subunit can bind to both alpha and beta receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.
Synonyms: PDGFRB, CD140B, EC 2.7.10.1, JTK12, PDGF-R-BETA, PDGFR, PDGFR1
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Specificity
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PDGFB (L48) pAb detects endogenous levels of PDGFB protein.
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Molecular Weight
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27 kDa
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Application Notes
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WB: 1:500-1:1000 IHC: 1:50-1:200
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE).
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Buffer
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1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
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Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "Platelet-Derived Growth Factor beta Polypeptide (Simian Sarcoma Viral (V-Sis) Oncogene Homolog) (PDGFB)", type "Antibodies"
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Hosts
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Rabbit (29), Goat (1)
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Reactivities
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Human (30), Rat (Rattus) (22), Mouse (Murine) (20), Cow (Bovine) (16), Dog (Canine) (16), Horse (Equine) (15), Rabbit (15), Sheep (Ovine) (15)
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Applications
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Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (9), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (4), Functional Studies (Func) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (3), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (3), AA 101-116 (2)
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