H/K/N-Ras antibody

Antigen: H/K/N-Ras

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN459697

Additional Information

Format: Liquid

Description: The KRAS gene encodes the human cellular homolog of a transforming gene isolated from the Kirsten rat sarcoma virus. The RAS proteins are GDP/GTP-binding proteins that act as intracellular signal transducers. The most well-studied members of the RAS (derived from 'RAt Sarcoma' virus) gene family include KRAS, HRAS, and NRAS. These genes encode immunologically related proteins with a molecular mass of 21 kD and are homologs of rodent sarcoma virus genes that have transforming abilities. While these wildtype cellular proteins in humans play a vital role in normal tissue signaling, including proliferation, differentiation, and senescence, mutated genes are potent oncogenes that play a role in many human cancers.
Synonyms: NRAS, N-RAS, NRAS1,HRAS, C-BAS/HAS, C-H-RAS, H-RAS-1, HRAS1, K-RAS, N-RAS, P21RAS, RASH1,HRAS, C-BAS/HAS, C-H-RAS, H-RAS-1, HRAS1, K-RAS, N-RAS, P21RAS, RASH1

Specificity: RASH/RASK (H27) pAb detects endogenous levels of RASH/RASK protein.

Molecular Weight: 25 kDa

Application Details

Application Notes: WB: 1:500-1:1000 IHC: 1:50-1:200

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

anti-H/K/N-Ras antibody