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Antibodies
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anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibody
Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibody
| Antigen |
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| Synonyms |
Darpp32, AU040756, DARPP-32, DARPP32, FLJ20940, Darpp-32, darpp32, darpp-32, PPP1R1B, MGC139723 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB) (32), Immunofluorescence (IF) (23), Flow Cytometry (FACS) (20), ELISA (7), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Enzyme Immunoassay (EIA) (3), Immunoprecipitation (IP) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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| Catalog no. |
ABIN459780 |
| Quantity |
100µg (1 mg/ml) (Variants) |
| Price |
743.71 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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DARPP-32
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Format
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Liquid
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Description
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Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson’s disease and drug abuse. DARPP-32 (for dopamine and adenosine 3’,5’-monophosphate-regulated phosphoprotein of 32 kDa) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.
Synonyms: Ppp1r1b, Darpp32, Darpp-32
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Specificity
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DARPP-32 (P69) pAb detects endogenous levels of DARPP-32 protein.
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Molecular Weight
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32 kDa
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Application Notes
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WB: 1:500-1:1000 IHC: 1:50-1:200
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE).
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Buffer
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1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
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Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)", type "Antibodies"
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Hosts
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Rabbit (48), Mouse (4)
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Reactivities
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Human (43), Rat (Rattus) (37), Mouse (Murine) (35), Cow (Bovine) (12), Dog (Canine) (7), Chicken (6), Rabbit (3), Monkey (2), Xenopus laevis (2), Pig (Porcine) (1)
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Applications
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Western Blotting (WB) (32), Immunofluorescence (IF) (23), Flow Cytometry (FACS) (20), ELISA (7), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Enzyme Immunoassay (EIA) (3), Immunoprecipitation (IP) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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pThr75 (8), pThr34 (6), Ab-34 (1), Ab-75 (1), N-Term (1)
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