»
Antibodies
»
anti-E1A Binding Protein P300 (EP300) antibody
E1A Binding Protein P300 (EP300) antibody
| Antigen |
|
| Synonyms |
p300, KAT3B, EP300 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
|
| Catalog no. |
ABIN459945 |
| Quantity |
100µg (1 mg/ml) (Variants) |
| Price |
743.71 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 7 to 10 Business Days |
|
Alternative name
|
p300
|
|
Format
|
Liquid
|
|
Description
|
Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A, bind to this element. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265 kDa nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.
Synonyms: Rabbit polyclonal to p300, p300 HAT, EC 2.3.1.48, E1A-associated protein p300, p300, P300
|
|
Specificity
|
P300 (E83) pAb detects endogenous levels of p300 protein.
|
|
Molecular Weight
|
265.0 kDa
|
|
Application Notes
|
IHC: 1:50-1:200 WB: 1:500-1:1000
|
|
Concentration
|
1 mg/ml
|
|
Purity
|
> 95% (by SDS-PAGE).
|
|
Purification
|
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|
|
Buffer
|
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
|
|
Storage
|
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "E1A Binding Protein P300 (EP300)", type "Antibodies"
|
Hosts
|
Rabbit (15), Mouse (12)
|
|
Reactivities
|
Human (23), Mouse (Murine) (3), Rat (Rattus) (3), Mink (2), Monkey (2)
|
|
Applications
|
Western Blotting (WB) (23), Enzyme Immunoassay (EIA) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunofluorescence (IF) (9), Immunoprecipitation (IP) (7), Dot Blot (Dot) (4), Immunohistochemistry (IHC) (4), ELISA (3), Electrophoretic Mobility-Shift Assay (EMSA) (1)
|
|
Epitopes
|
pSer1834 (2), pSer89 (2), N-Term (1), S1834 (1), S89 (1)
|
