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P21 Protein (Cdc42/Rac)-Activated Kinase 1/2/3 (PAK1/2/3) antibody
| Antigen | P21 Protein (Cdc42/Rac)-Activated Kinase 1/2/3 (PAK1/2/3) |
| Clonality | Polyclonal |
| Host |
 Alternatives Rabbit |
| Reactivity |
Alternatives Human, Rat (Rattus) |
| Application |
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
|
| Catalog no. | ABIN459953 |
| Quantity | 100µg (1 mg/ml) (Variants) |
| Price | 743.71 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Alternative name | PAK1/2/3 |
| Format | Liquid |
| Description |
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is Mek kinase, an upstream effector of Mek4 which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTPdependent manner with Rac1 and Cdc42, they do not interact with Rho. Synonyms: EC 2.7.11.1, p21-activated kinase 2, PAK-2, Gamma-PAK, PAK65, S6/H4 kinase, p58, PAK2 kinase |
| Specificity | PAK1/2/3 (E417/396/415) pAb detects endogenous levels of PAK1/2/3 protein. |
| Molecular Weight | 62 kDa |
Application Details
| Application Notes | WB: 1:500-1:1000 IHC: 1:50-1:200 |
| Concentration | 1 mg/ml |
| Purity | > 95% (by SDS-PAGE). |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Buffer | 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Restrictions | For Research Use only |
Images
| anti-P21 Protein (Cdc42/Rac)-Activated Kinase 1/2/3 (PAK1/2/3) antibody anti-P21 Protein (Cdc42/Rac)-Activated Kinase 1/2/3 (PAK1/2/3) antibody (Image 2) |
Alternatives
Alternatives for antigen "P21 Protein (Cdc42/Rac)-Activated Kinase 1/2/3 (PAK1/2/3)", type "Antibodies"
| Hosts | Rabbit (11) |
| Reactivities | Human (10), Rat (Rattus) (10), Mouse (Murine) (8), Cow (Bovine) (2), Dog (Canine) (2) |
| Applications | Immunohistochemistry (IHC) (6), Western Blotting (WB) (5), ELISA (4), Immunofluorescence (IF) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2) |
| Epitopes | pThr402 (3), pThr423/402/421 (2), pSer144 (1), pThr423 (1) |
