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zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody

Antigen

zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)
Synonyms SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Western Blotting (WB)
Catalog no. ABIN459993
Quantity 100µg  (1 mg/ml)  (Variants)
Price 743.71 $   Plus shipping costs $35.00
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anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody

Additional Information
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Alternative name ZAP-70
Format Liquid
Description The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and a zeta-containing homodimer or heterodimer. The disulfide-linked Ti alpha-beta heterodimer is responsible for antigen recognition, but the short five amino acid cytoplasmic domains of Ti alpha and beta are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and zeta subunits suggest a role in signal transduction. Of these, the zeta chain, which is expressed as either a homodimer or heterodi-mer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A 70 kDa tyrosine phosphoprotein, ZAP-70, has been identified that associates with zeta and undergoes tyrosine phosphorylation following TCR stimulation.
Synonyms: ZAP70, EC 2.7.10.2, SRK, STD, TZK, ZAP-70
Specificity ZAP-70 (T286) pAb detects endogenous levels of ZAP-70 protein.
Molecular Weight 70 kDa

Application Details
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Application Notes WB: 1:500-1:1000
Concentration 1 mg/ml
Purity > 95% (by SDS-PAGE).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", type "Antibodies"
Hosts Rabbit (99), Mouse (42)
Reactivities Human (136), Mouse (Murine) (66), Rat (Rattus) (60), Cow (Bovine) (18), Pig (Porcine) (18), Dog (Canine) (17), Horse (Equine) (16), Rabbit (16), Chicken (15), Guinea Pig (15)
Applications Western Blotting (WB) (78), Flow Cytometry (FACS) (37), Immunohistochemistry (IHC) (33), Immunofluorescence (IF) (32), ELISA (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27), Enzyme Immunoassay (EIA) (12), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (6), FITC (6), PE (3), HRP (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alkaline Phosphatase (AP) (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
Epitopes pTyr319 (10), pTyr493 (8), Ab-319 (5), Ab-493 (5), C-Term (5), Center (3), Ab-292 (1), Ab-315 (1), pTyr292 (1), pTyr315 (1), pTyr492 (1)