V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) antibody

Antigen: V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3)

Synonyms: ERBB3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Catalog no.: ABIN460032

Additional Information

Alternative name: ErbB-3

Format: Liquid

Description: The ErbB3 gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. ErbB3 is a membrane-bound protein which has a neuregulin binding domain but not an active kinase domain. It can therefore bind this ligand but cannot convey a signal into the cell via protein phosphorylation. However it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers including prostate, bladder and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported but they have not been thoroughly characterized.
Synonyms: IgG Fraction of Anti-Human HER3 (Rabbit), c-erbB3, EC 2.7.10.1, Tyrosine kinase-type cell surface receptor HER3, ERBB3, HER3

Specificity: ErbB-3 (S1285) pAb detects endogenous levels of ErbB-3 protein.

Molecular Weight: 148 kDa

Application Details

Application Notes: WB: 1:500-1:1000 IHC: 1:50-1:200 IF:1:50-1:200

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) antibody

anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) antibody (Image 2)