Histone H1 antibody
| Antigen |
|
| Synonyms |
H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Immunofluorescence (IF) (35), Western Blotting (WB) (27), Flow Cytometry (FACS) (18), ELISA (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunohistochemistry (IHC) (9), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunocytochemistry (ICC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
|
| Catalog no. |
ABIN460034 |
| Quantity |
100µg (1 mg/ml) (Variants) |
| Price |
743.71 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 7 to 10 Business Days |
|
Format
|
Liquid
|
|
Description
|
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.The C-terminal domain is the primary determinant of histone H1 binding to chromatin in vivo. The C-terminal region also shows the most differences in aa sequence between the H1 histone variants H1.0 kDa/1/2/3/4/5.
Synonyms: H1F0, H1, H10, H1FV, MGC5241
|
|
Specificity
|
Histone H1 (P18) pAb detects endogenous levels of Histone H1 protein.
|
|
Molecular Weight
|
21 kDa
|
|
Application Notes
|
WB: 1:500-1:1000 IHC: 1:50-1:200 IF:1:50-1:200
|
|
Concentration
|
1 mg/ml
|
|
Purity
|
> 95% (by SDS-PAGE).
|
|
Purification
|
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|
|
Buffer
|
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
|
|
Storage
|
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Histone H1", type "Antibodies"
|
Hosts
|
Rabbit (27), Mouse (18), Chicken (4), Sheep (4)
|
|
Reactivities
|
Human (42), Cow (Bovine) (21), Mouse (Murine) (21), Rat (Rattus) (17), Dog (Canine) (16), Horse (Equine) (16), Rabbit (16), Pig (Porcine) (15)
|
|
Applications
|
Immunofluorescence (IF) (35), Western Blotting (WB) (27), Flow Cytometry (FACS) (18), ELISA (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (15), Immunohistochemistry (IHC) (9), Immunoprecipitation (IP) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunocytochemistry (ICC) (3), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
Acetyl-Lys25 (1), pThr17 (1)
|
