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Antibodies
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anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) antibody
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) antibody
| Antigen |
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| Synonyms |
Crkol, AA589403, AI325100, 1110025F07Rik, CRKL, MGC63469, zgc:63469, wu:fa55g05, wu:fj60e01 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
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| Catalog no. |
ABIN460079 |
| Quantity |
100µg (1 mg/ml) (Variants) |
| Price |
743.71 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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CrkL
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Format
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Liquid
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Description
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SH2 and SH3 (Src homology) domains were originally identified as critical functional domains within non-receptor proteins with tyrosine kinase activity. A subset of these proteins appears to exist predominately of SH2/SH3 domains in the absence of detectable catalytic domains. One of the first members of the family to be identified, Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa.
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Specificity
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CrkL (P201) pAb detects endogenous levels of CrkL protein.
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Molecular Weight
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39 kDa
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Application Notes
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WB: 1:500-1:1000 IHC: 1:50-1:200 IF:1:50-1:200
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE).
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Buffer
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1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
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Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL)", type "Antibodies"
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Hosts
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Rabbit (11), Mouse (6), Goat (5)
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Reactivities
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Human (19), Mouse (Murine) (5), Rat (Rattus) (5)
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Applications
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Western Blotting (WB) (19), Immunohistochemistry (IHC) (7), ELISA (6), ELISA (Detection) (3), Enzyme Immunoassay (EIA) (3), Immunofluorescence (IF) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Flow Cytometry (FACS) (1), Immunoprecipitation (IP) (1)
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Epitopes
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pTry207 (13)
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