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Vimentin (VIM) antibody

Antigen

Vimentin (VIM)
Synonyms FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Catalog no. ABIN460131
Quantity 100µg  (1 mg/ml)  (Variants)
Price 743.71 $   Plus shipping costs $35.00
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anti-Vimentin (VIM) antibody

anti-Vimentin (VIM) antibody (2)

Additional Information
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Alternative name Vimentin
Format Liquid
Description Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, alpha-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.
Synonyms: Monoclonal Rabbit Antibody to Human Vimentin, Vimentin, 7431, VIM, 193060, P08670, FLJ36605,
Specificity Vimentin (L52) pAb detects endogenous levels of Vimentin protein
Molecular Weight 54 kDa

Application Details
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Application Notes WB: 1:500-1:1000 IHC: 1:50-1:200 IF:1:50-1:200
Concentration 1 mg/ml
Purity > 95% (by SDS-PAGE).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Images Secondary Antibodies Variants back to top

Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
Hosts Rabbit (94), Mouse (74), Chicken (9), Sheep (3), Guinea Pig (2), Goat (1)
Reactivities Human (156), Rat (Rattus) (66), Mouse (Murine) (60), Chicken (32), Cow (Bovine) (28), Pig (Porcine) (23), Dog (Canine) (9), Mammalian (8), Monkey (6), Goat (5), Hamster (5), Amphibian (4), Potoroo (2), Fish (1), Primate (1), Rabbit (1), Xenopus laevis (1)
Applications Western Blotting (WB) (105), Immunofluorescence (IF) (73), Flow Cytometry (FACS) (57), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (57), Immunohistochemistry (IHC) (54), ELISA (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (29), Enzyme Immunoassay (EIA) (16), Immunoprecipitation (IP) (15), Immunocytochemistry (ICC) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Dot Blot (Dot) (2), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Transfected cell culture (TCC) (2)
Conjugates FITC (10), Biotin (8), Alexa Fluor 488 (4), PE (4), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
Epitopes pSer82 (21), C-Term (2), Center (2), S82 (2), Ab-56 (1), Ab-83 (1), pSer56 (1), pSer83 (1)