CD82 (CD83) antibody
| Antigen |
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| Synonyms |
BL11, HB15, R2, 4F9, C33, IA4, ST6, GR15, KAI1, SAR2, TSPAN27 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
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| Catalog no. |
ABIN460374 |
| Quantity |
100µg (1 mg/ml) (Variants) |
| Price |
743.71 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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CD83
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Format
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Liquid
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Description
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CD83 is a heavily glycosylated membrane protein of the immunoglobulin (Ig) superfamily that is expressed in mature dendritic cells, Langerhans cells and interdigitating reticulum cells within lymphoid tissues. Structurally, CD83 resembles other Ig superfamily members, which have an extracellular V-type Ig-like domain, a single transmembrane domain and a 40 residue cytoplasmic tail. CD83 expression is used as a marker for mature, antigen presenting dendritic cells that are capable of generating tumor-specific T cell immunity, a phenotype with implications as an anticancer vaccine. CD83-IgG1(fc) chimera studies indicate that CD83 is a sialic acid-binding, Ig-like lectin (Siglec) adhesion molecule that is involved in cell adhesion/signaling by hosting dendritic cell interactions with monocytes and CD8+ T cells.
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Specificity
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CD83 (P130) pAb detects endogenous levels of CD83 protein
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Molecular Weight
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23 kDa
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Application Notes
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WB: 1:500-1:1000 IHC: 1:50-1:200
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE).
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Buffer
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1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
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Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "CD82 (CD83)", type "Antibodies"
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Hosts
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Mouse (41), Rabbit (11), Rat (5)
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Reactivities
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Human (50), Mouse (Murine) (9), Primate (3), Rat (Rattus) (3)
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Applications
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Flow Cytometry (FACS) (35), Western Blotting (WB) (19), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunohistochemistry (IHC) (5), ELISA (3), ELISA (Detection) (3), Immunofluorescence (IF) (2), Immunoprecipitation (IP) (1)
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Conjugates
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FITC (7), PE (6), Biotin (4), RPE (1)
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Epitopes
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C-Term (3)
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