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Actin-beta antibody
| Antigen | Actin-beta |
| Clonality | Polyclonal |
| Host |
Rabbit |
| Reactivity |
Human, Rat (Rattus) |
| Application |
Western Blotting (WB), Immunohistochemistry (IHC)
|
| Catalog no. | ABIN460395 |
| Quantity | 100µg (1 mg/ml) |
| Price | 743.71 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Format | Liquid |
| Description | Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Be aware when using beta actin antibodies as loading controls that although beta actin is normally the best protein to use as a loading control, under some conditions its amounts can vary between samples. In particular, expression of actin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) in cells undergoing adipose differentiation actin synthesis decreases by 90%. |
| Specificity | Actin-beta (D2) pAb detects endogenous levels of Actin-beta protein. |
| Molecular Weight | 42 kDa |
Application Details
| Application Notes | WB: 1:500-1:1000 IHC: 1:50-1:200 |
| Concentration | 1 mg/ml |
| Purity | > 95% (by SDS-PAGE). |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Buffer | 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Restrictions | For Research Use only |
