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Antibodies
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anti-Laminin, beta 1 (LAMB1) antibody
Laminin, beta 1 (LAMB1) antibody
| Antigen |
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| Synonyms |
CLM, MGC142015, gup, fc20b05, sb:cb95, wu:fc20b05, Lamb1-1, LAMB1, lams, lamb2 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Immunofluorescence (IF) (29), Western Blotting (WB) (27), Flow Cytometry (FACS) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Immunoprecipitation (IP) (13), ELISA (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Enzyme Immunoassay (EIA) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN460490 |
| Quantity |
100µg (1 mg/ml) (Variants) |
| Price |
743.71 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Alternative name
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Laminin beta-1
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Format
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Liquid
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Description
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Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing type IV collagen networks, nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble and bind to other matrix macromolecules, and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.
Synonyms: LAMA1, LAMA
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Specificity
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Laminin beta-1 (D1742) pAb detects endogenous levels of Laminin beta-1 protein.
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Molecular Weight
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220 kDa
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Application Notes
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WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE).
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Buffer
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1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
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Storage
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Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "Laminin, beta 1 (LAMB1)", type "Antibodies"
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Hosts
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Rabbit (26), Rat (11), Mouse (7)
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Reactivities
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Mouse (Murine) (33), Human (31), Rat (Rattus) (20), Chicken (15), Dog (Canine) (15), Horse (Equine) (15), Rabbit (15)
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Applications
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Immunofluorescence (IF) (29), Western Blotting (WB) (27), Flow Cytometry (FACS) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (14), Immunoprecipitation (IP) (13), ELISA (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Enzyme Immunoassay (EIA) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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Biotin (4), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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