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Hepatocyte Nuclear Factor 4, alpha (HNF4A) antibody

Antigen

Hepatocyte Nuclear Factor 4, alpha (HNF4A)
Synonyms HNF4A, HNF4alpha, HNF-4alpha, DmHNF4, HNF-4, HNF-4(D), HNF4, Hnf-4h, NR2A4, dHNF-4, dHNF4, DmelCG9310, CG9310
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Catalog no. ABIN460743
Quantity 100µg  (1 mg/ml)  (Variants)
Price 939.71 $   Plus shipping costs $45.00
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anti-Hepatocyte Nuclear Factor 4, alpha (HNF4A) antibody

Additional Information
Application Details Images Alternatives Variants back to top

Alternative name HNF4alpha
Format Liquid
Description HNF-1 (alpha and beta), HNF-3 (alpha, beta and gamma), HNF-4 (alpha and gamma), and HNF-6 compose, in part, a homoeprotein family designated the Hepatocyte Nuclear Factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. HNF-3alpha, HNF-3beta and HNF-3gamma regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3alpha and HNF-3beta have also been shown to be involved in gastrulation events such as body axis formation. HNF-4alpha and HNF-4gamma have been shown to be important for early embryo development. HNF-4alpha is expressed in liver, kidney, pancreas, small intestine, testis and colon, and HNF-4gamma is expressed in each of these tissues except liver. HNF-6 has been shown to bind to the promoter of HNF-3beta, which indicates a potential role of HNF-6 in gut endoderm epithelial cell differentiation. Evidence suggests that HNF-6 may also be a transriptional activator for at least 22 other hepatocyte-enriched genes, including cytochrome P450 2C13 and alpha-1 antitrypsin.
Specificity HNF4alpha (K298) pAb detects endogenous levels of HNF4alpha protein.
Molecular Weight 52 kDa

Application Details
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Application Notes WB: 1:500-1:1000 IHC: 1:50-1:200
Concentration 1 mg/ml
Purity > 95% (by SDS-PAGE).
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Images Variants back to top

Alternatives for antigen "Hepatocyte Nuclear Factor 4, alpha (HNF4A)", type "Antibodies"
Hosts Rabbit (84), Goat (5), Mouse (5)
Reactivities Human (90), Rat (Rattus) (54), Mouse (Murine) (53), Cow (Bovine) (40), Pig (Porcine) (40), Chicken (39), Guinea Pig (36), Horse (Equine) (36), Rabbit (18), Zebrafish (Danio rerio) (18), Dog (Canine) (5), Xenopus laevis (3), Zebrafish (2)
Applications Western Blotting (WB) (57), ELISA (44), Immunofluorescence (IF) (37), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (IHC) (6), Immunocytochemistry (ICC) (3), Immunoelectron Microscopy (IEM) (2), Immunoprecipitation (IP) (2), Chromatin Immunoprecipitation (ChiP) (1), Dot Blot (DB) (1), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1)
Conjugates Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
Epitopes pSer304 (20), pSer313 (20), Internal Region (7), N-Term (5), pSer142 (3), Center (2), Ser142 (2), Gly162 (1), N-Term,AA 116-145 (1), Ser304 (1)