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Heat Shock 27kDa Protein 1 (HSPB1) antibody
| Antigen | Heat Shock 27kDa Protein 1 (HSPB1) |
| Synonyms |
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, M ...
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| Clonality | Polyclonal |
| Host |
 Alternatives Rabbit |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
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| Catalog no. | ABIN461082 |
| Quantity | 100µg (1 mg/ml) (Variants) |
| Price | 743.71 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Alternative name | HSP27 |
| Format | Liquid |
| Description | Hsp27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins (HSP) produced by all organisms studied. Hsp27 synthesis is induced by elevated temperature, as well as estrogen in hormone responsive cells. This protein is involved in stress resistance and actin organization. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSP's and mammalian a-crystalline lens protein. Because of the estrogen-responsive nature of Hsp27, this protein has been studied extensively in human estrogen-responsive tissues such as cervix, endometrium and breast tissue. This work has led to the suggestion that Hsp27 may be a useful marker in classifying various hormone sensitive tumors. |
| Specificity | HSP27 (A72) pAb detects endogenous levels of HSP27 protein. |
| Molecular Weight | 27 kDa |
| Synonyms | CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, MGC147259 |
Application Details
| Application Notes | WB: 1:500-1:1000 IHC: 1:50-1:200 |
| Concentration | 1 mg/ml |
| Purity | > 95% (by SDS-PAGE). |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Buffer | 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Restrictions | For Research Use only |
Images
| anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (Image 2) |
Alternatives
Alternatives for antigen "Heat Shock 27kDa Protein 1 (HSPB1)", type "Antibodies"
| Hosts | Rabbit (126), Mouse (73), Chicken (1) |
| Reactivities | Human (188), Rat (Rattus) (28), Mouse (Murine) (22), Dog (Canine) (10), Chicken (6), Cow (Bovine) (6), Monkey (5), Mammalian (4), Cat (Feline) (3), Fish (3), Goat (3), Guinea Pig (3), Hamster (3), Pig (Porcine) (3), Horse (Equine) (2) |
| Applications | Western Blotting (WB) (184), Immunohistochemistry (IHC) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (61), ELISA (43), Immunofluorescence (IF) (38), Immunoprecipitation (IP) (37), Enzyme Immunoassay (EIA) (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunocytochemistry (ICC) (15), Flow Cytometry (FACS) (13), Dot Blot (Dot) (4), Immunoelectron Microscopy (IEM) (2), ELISA (Detection) (1), Radioimmunoassay (RIA) (1) |
| Conjugates | Biotin (7), FITC (7), DY488 (1), PE (1) |
| Epitopes | pSer82 (13), pSer78 (12), pSer15 (11), p (4), S78 (2), pSer83 (2), Center (1), S15 (1), S82 (1), S83 (1), pS83 (1), pSer78/82 (1), pSer85 (1) |
