Gap Junction Protein, alpha 1, 43kd (GJA1) antibody

Antigen: Gap Junction Protein, alpha 1, 43kd (GJA1)

Synonyms: CX43, GJAL, ODDD, DFNB38, Cx43, Npm1, Cnx43, Gja-1, AU042049, AW546267, Cx43alpha1, connexin43, Cx32, Cnx32, Gjb-1, MGC6217, AI118175, MGC93610, gja1, cx43a1, zfCx43.3, MGC128721, gja1-A, connexin 43, cx43, gjal, oddd, dfnb38, CONNEXIN 43

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN461090

Additional Information

Alternative name: Connexin 43

Format: Liquid

Description: The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. In Src transformed cells, there is a decrease in gap junctional communication, which appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on Tyr 265, resulting in a stable interaction between both proteins, which leads to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the ab-sence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as c-Src and/or pp60v-Src. In human vascular endothelial cells, connexin 43 is posttranslationally modified during mitosis. Mitosis-specific phosphorylation of connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of connexin 43.

Specificity: Connexin 43 (R361) pAb detects endogenous levels of Connexin 43 protein.

Molecular Weight: 43 kDa

Synonyms: CX43, GJAL, ODDD, DFNB38, Cx43, Npm1, Cnx43, Gja-1, AU042049, AW546267, Cx43alpha1, connexin43, Cx32, Cnx32, Gjb-1, MGC6217, AI118175, MGC93610, gja1, cx43a1, zfCx43.3, MGC128721, gja1-A, connexin 43, cx43, gjal, oddd, dfnb38, CONNEXIN 43

Application Details

Application Notes: WB: 1:500-1:1000 IHC: 1:50-1:200

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

anti-Gap Junction Protein, alpha 1, 43kd (GJA1) antibody

anti-Gap Junction Protein, alpha 1, 43kd (GJA1) antibody (Image 2)