ZA70 (pTyr493) antibody

Antigen: ZA70

Epitope: pTyr493

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN461441

Additional Information

Format: Liquid

Description: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and a zeta-containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR zeta chain through two Src homology (SH2) domains. This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70 function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.

Specificity: P-ZAP-70 (Y493) pAb detects endogenous levels of ZAP-70 protein only when phosphorylated at Y493.

Molecular Weight: 70 kDa

Application Details

Application Notes: WB: 1:500-1:1000 IHC: 1:50-1:200

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Buffer: 1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

anti-ZA70 (pTyr493) antibody

anti-ZA70 (pTyr493) antibody (Image 2)