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Heat Shock 27kDa Protein 1 (HSPB1) (pSer82) antibody
| Antigen | Heat Shock 27kDa Protein 1 (HSPB1) |
| Synonyms |
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, M ...
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| Epitope |
 Alternatives pSer82 |
| Clonality | Polyclonal |
| Host |
Alternatives Rabbit |
| Reactivity |
Alternatives Human, Rat (Rattus) |
| Application |
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
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| Catalog no. | ABIN461468 |
| Quantity | 100µg (1 mg/ml) |
| Price | 743.71 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Alternative name | HSP27 |
| Format | Liquid |
| Description | HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. The intracellular concentration of HSP 27 increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. In addition to heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II. |
| Specificity | P-HSP27 (S82) pAb detects endogenous levels of p-HSP27 protein. |
| Molecular Weight | 27.0 kDa |
| Synonyms | CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322, MKBP, Hs.78846, LOH11CR1K, MGC133245, 27kDa, Hsp27, hsp1, cb153, hsp25, hsp27, zgc:103437, MGC85307, HSPB1, cmt2f, hsp28, hs.76067, MGC147259 |
Application Details
| Application Notes | WB: 1:500-1:1000 IHC: 1:50-1:200 |
| Concentration | 1 mg/ml |
| Purity | > 95% (by SDS-PAGE). |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Buffer | 1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Restrictions | For Research Use only |
Images
| anti-Heat Shock 27kDa Protein 1 (HSPB1) (pSer82) antibody anti-Heat Shock 27kDa Protein 1 (HSPB1) (pSer82) antibody (Image 2) |
Alternatives
Alternatives for antigen "Heat Shock 27kDa Protein 1 (HSPB1)", type "Antibodies"
| Hosts | Rabbit (126), Mouse (73), Chicken (1) |
| Reactivities | Human (188), Rat (Rattus) (27), Mouse (Murine) (21), Dog (Canine) (10), Chicken (6), Cow (Bovine) (6), Monkey (5), Mammalian (4), Cat (Feline) (3), Fish (3), Goat (3), Guinea Pig (3), Hamster (3), Pig (Porcine) (3), Horse (Equine) (2) |
| Applications | Western Blotting (WB) (184), Immunohistochemistry (IHC) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (61), ELISA (43), Immunofluorescence (IF) (38), Immunoprecipitation (IP) (37), Enzyme Immunoassay (EIA) (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunocytochemistry (ICC) (15), Flow Cytometry (FACS) (13), Dot Blot (Dot) (4), Immunoelectron Microscopy (IEM) (2), ELISA (Detection) (1), Radioimmunoassay (RIA) (1) |
| Conjugates | Biotin (7), FITC (7), DY488 (1), PE (1) |
| Epitopes | pSer82 (13), pSer78 (12), pSer15 (11), p (3), S78 (2), pSer83 (2), Center (1), S15 (1), S82 (1), S83 (1), pS83 (1), pSer78/82 (1), pSer85 (1) |
