ABTB1 antibody (AA 465-478)

Catalog No. ABIN461922

Product Details anti-ABTB1 Antibody

Target: ABTB1 (Ankyrin Repeat and BTB (POZ) Domain Containing 1 (ABTB1))

Binding Specificity: AA 465-478

Reactivity: Human, Mouse, Dog, Hamster, Horse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ABTB1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ABTB1 / BPOZ. This antibody is expected to recognise all reported isoforms (NP_115937.1, NP_742024.1, NP_742025.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Hamster, Panda, Dog, Horse (100%) Gibbon, Rat, Bat (93%) Elephant, Bovine (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide RALEDLLVSIGLDC from the C-terminus of human ABTB1 / BPOZ (NP_115937.1, NP_742024.1, NP_742025.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Hamster, Panda, Dog, Horse (100%), Gibbon, Rat, Bat (93%), Elephant, Bovine (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ABTB1

Target: ABTB1 (Ankyrin Repeat and BTB (POZ) Domain Containing 1 (ABTB1))

Alternative Name: BPOZ / ABTB1 (ABTB1 Products)

Synonyms: abtb1 antibody, MGC80260 antibody, ABTB1 antibody, MGC122091 antibody, BPOZ antibody, BTB3 antibody, BTBD21 antibody, EF1ABP antibody, AI847549 antibody, BC003234 antibody, ankyrin repeat and BTB domain containing 1 antibody, ankyrin repeat and BTB (POZ) domain containing 1 S homeolog antibody, ankyrin repeat and BTB (POZ) domain containing 1 antibody, ABTB1 antibody, abtb1.S antibody, abtb1 antibody, Abtb1 antibody

Background: Name/Gene ID: ABTB1

Synonyms: ABTB1, BPOZ, BTB3, EF1ABP, PP2259, BTBD21

Gene ID: 80325

NCBI Accession: NP_115937, NP_742024, NP_742025

UniProt: Q969K4