ZIC5 antibody (AA 651-663)

Catalog No. ABIN461927

Product Details anti-ZIC5 Antibody

Target: ZIC5 (Zinc Family Member 5 (ZIC5))

Binding Specificity: AA 651-663

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This ZIC5 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ZIC5.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Hamster, Elephant, Rabbit (92%) Mouse, Rat, Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EIYGNPEVVRTIH from the C-terminus of human ZIC5 (NP_149123.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Hamster, Elephant, Rabbit (92%), Mouse, Rat, Dog (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:128000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Not recommended for: WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ZIC5

Target: ZIC5 (Zinc Family Member 5 (ZIC5))

Alternative Name: ZIC5 (ZIC5 Products)

Synonyms: XZic5 antibody, XlZic5 antibody, opl antibody, zic-1 antibody, zic1 antibody, zic5-A antibody, 1700049L20Rik antibody, Opr antibody, Zic family member 5 antibody, Zic family member 5 S homeolog antibody, zinc finger protein ZIC 5 antibody, zinc finger protein of the cerebellum 5 antibody, ZIC5 antibody, zic5.S antibody, Zic5 antibody, LOC787451 antibody

Background: Name/Gene ID: ZIC5
Family: Zinc Finger

Synonyms: ZIC5, Zic family member 5, Zinc finger protein ZIC 5, Zinc family member 5 protein

Gene ID: 85416

NCBI Accession: NP_149123

UniProt: Q96T25

Pathways: Tube Formation