PTBP1 antibody (AA 2-14)

Catalog No. ABIN461929

Product Details anti-PTBP1 Antibody

Target: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Binding Specificity: AA 2-14

Reactivity: Human, Mouse, Rat, Cow, Pig, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This PTBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PTBP1. This antibody is expected to recognise all 4 reported isoforms (represented by NP_002810.1, NP_114367.1, NP_114368.1, NP_787041.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Bovine, Pig (100%) Horse, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide DGIVPDIAVGTKR-C from the N-terminus of human PTBP1 (NP_002810.1, NP_114367.1, NP_114368.1, NP_787041.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Hamster, Bovine, Pig (100%), Horse, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:8000), WB (1:8000, 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PTBP1

Target: PTBP1 (Polypyrimidine Tract Binding Protein 1 (PTBP1))

Alternative Name: PTBP1 (PTBP1 Products)

Synonyms: HNRNP-I antibody, HNRNPI antibody, HNRPI antibody, PTB antibody, PTB-1 antibody, PTB-T antibody, PTB2 antibody, PTB3 antibody, PTB4 antibody, pPTB antibody, Ptb antibody, hnrpi antibody, hnrnp-I antibody, vgrbp60 antibody, AA407203 antibody, AL033359 antibody, Pybp antibody, Pybp1 antibody, Pybp2 antibody, ptbp1 antibody, wu:fb99f12 antibody, zgc:110689 antibody, ptb antibody, ATPTB1 antibody, POLYPYRIMIDINE TRACT-BINDING antibody, POLYPYRIMIDINE TRACT-BINDING PROTEIN 1 antibody, T4P13.16 antibody, T4P13_16 antibody, polypyrimidine tract-binding protein 1 antibody, PTBP1 antibody, polypyrimidine tract binding protein 1 antibody, polypyrimidine tract binding protein 1 S homeolog antibody, polypyrimidine tract-binding protein antibody, polypyrimidine tract binding protein 1a antibody, polypyrimidine tract binding protein 1 L homeolog antibody, polypyrimidine tract-binding protein 1 antibody, PTBP1 antibody, ptbp1.S antibody, NAEGRDRAFT_80143 antibody, LOC100285404 antibody, Ptbp1 antibody, ptbp1a antibody, ptbp1.L antibody, PTB1 antibody, LOC100061673 antibody

Background: Name/Gene ID: PTBP1

Synonyms: PTBP1, HnRNP I, HNRNPI, HNRPI, PTB, PPTB, PTB2, PTB4, RNA-binding protein, HNRNP-I, PTB-1, PTB-T, PTB3

Gene ID: 5725

NCBI Accession: NP_002810, NP_114367, NP_114368, NP_787041

UniProt: P26599

Pathways: Regulation of Muscle Cell Differentiation