GPX4 antibody (AA 184-196)

Catalog No. ABIN461940

Product Details anti-GPX4 Antibody

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Binding Specificity: AA 184-196

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GPX4. This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Goat, Hamster, Bovine (92%) Marmoset, Mouse, Rat, Pig, Opossum, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EEPLVIEKDLPHY from the C-terminus of human GPX4 (NP_002076.2, NP_001034937.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Goat, Hamster, Bovine (92%), Marmoset, Mouse, Rat, Pig, Opossum, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000), WB (1:16000, 0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: GPX4 / MCSP (GPX4 Products)

Synonyms: GPx-4 antibody, GSHPx-4 antibody, MCSP antibody, PHGPx antibody, snGPx antibody, snPHGPx antibody, Phgpx antibody, gpx-4 antibody, snGpx antibody, cb563 antibody, cb691 antibody, PHGPxa antibody, sb:cb563 antibody, zgc:92253 antibody, wu:fb82e03 antibody, 1700027O09Rik antibody, mtPHGPx antibody, ATGPX4 antibody, F11L15.5 antibody, glutathione peroxidase 4 antibody, GP-GPx4 antibody, glutathione peroxidase 4 antibody, glutathione peroxidase 4a antibody, GPX4 antibody, Gpx4 antibody, gpx4a antibody

Background: Name/Gene ID: GPX4

Synonyms: GPX4, GPx-4, MCSP, SnPHGPx, Phospholipid hydroperoxidase, SnGPx, Glutathione peroxidase 4, GSHPx-4, PHGPx

Gene ID: 2879

NCBI Accession: NP_002076, NP_001034937

UniProt: P36969