UCP2 antibody (AA 101-113)

Catalog No. ABIN461943

Product Details anti-UCP2 Antibody

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Binding Specificity: AA 101-113

Reactivity: Human, Mouse, Rat, Dog, Bat, Cow, Hamster, Horse, Monkey, Pig, Rabbit, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This UCP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human UCP2.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Xenopus, Stickleback (100%) Smelt, Zebrafish (92%) Trout, Salmon (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DSVKQFYTKGSEH from an internal region of human UCP2 (NP_003346.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Xenopus, Stickleback (100%), Smelt, Zebrafish (92%), Trout, Salmon (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for UCP2

Target: UCP2 (Uncoupling Protein 2 (Mitochondrial, Proton Carrier) (UCP2))

Alternative Name: UCP2 (UCP2 Products)

Synonyms: UCP2 antibody, DKFZp470P1633 antibody, ucp2 antibody, MGC53319 antibody, MGC75881 antibody, cb74 antibody, ATUCP2 antibody, K19M22.21 antibody, K19M22_21 antibody, uncoupling protein 2 antibody, BMIQ4 antibody, SLC25A8 antibody, UCPH antibody, Slc25a8 antibody, mitochondrial uncoupling protein 2 antibody, uncoupling protein 2 antibody, uncoupling protein 2 (mitochondrial, proton carrier) L homeolog antibody, uncoupling protein 2 (mitochondrial, proton carrier) antibody, uncoupling protein 1 antibody, PTRG_09289 antibody, UCP2 antibody, LOC100282746 antibody, ucp2.L antibody, ucp2 antibody, ucp1 antibody, Ucp2 antibody

Background: Name/Gene ID: UCP2
Subfamily: Mitochondrial carrier
Family: Transporter

Synonyms: UCP2, BMIQ4, UCP 2, Uncoupling protein 2, UCPH, SLC25A8

Gene ID: 7351

NCBI Accession: NP_003346

UniProt: P55851

Pathways: Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Proton Transport