RPL8 antibody (AA 181-195)

Catalog No. ABIN461948

Product Details anti-RPL8 Antibody

Target: RPL8 (Ribosomal Protein L8 (RPL8))

Binding Specificity: AA 181-195

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Rabbit, Hamster, Monkey, Bat, Chicken, Pig, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This RPL8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RPL8. The reported variants represent identical protein (NP_000964.1 and NP_150644.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Smelt, Stickleback, Medaka, Pufferfish, Zebrafish, Sea anemone, Ant, Water flea (100%) Catfish, Mosquito (93%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide KAGRAYHKYKAKRNC from an internal region of human RPL8 (NP_000964.1, NP_150644.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Smelt, Stickleback, Medaka, Pufferfish, Zebrafish, Sea anemone, Ant, Water flea (100%), Catfish, Mosquito (93%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (1:128000, 0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RPL8

Target: RPL8 (Ribosomal Protein L8 (RPL8))

Alternative Name: RPL8 / L8 (RPL8 Products)

Synonyms: L8 antibody, zgc:73105 antibody, zgc:77641 antibody, CG1263 antibody, Dmel\\CG1263 antibody, M(3)62F antibody, M(3)LS2 antibody, Rp L8 antibody, anon-EST:Posey5 antibody, rpL8 antibody, RPL8 antibody, ribosomal protein L8 antibody, ribosomal protein L8 S homeolog antibody, Ribosomal protein L8 antibody, microRNA 6850 antibody, RPL8 antibody, Rpl8 antibody, rpl8.S antibody, rpl8 antibody, RpL8 antibody, MIR6850 antibody

Background: Name/Gene ID: RPL8

Synonyms: RPL8, 60S ribosomal protein L8, Ribosomal protein L8, L8

Gene ID: 6132

NCBI Accession: NP_000964, NP_150644

UniProt: P62917

Pathways: Regulation of Cell Size