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P4HA1 antibody (AA 431-443)

P4HA1 Reactivity: Human, Dog, Pig, Cow, Horse, Rabbit, Monkey, Chicken, Bat WB, ELISA, IHC, IHC (p) Host: Goat Polyclonal unconjugated
Catalog No. ABIN461984
  • Target See all P4HA1 Antibodies
    P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))
    Binding Specificity
    • 8
    • 8
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 431-443
    Reactivity
    • 32
    • 10
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Human, Dog, Pig, Cow, Horse, Rabbit, Monkey, Chicken, Bat
    Host
    • 29
    • 4
    • 1
    Goat
    Clonality
    • 32
    • 2
    Polyclonal
    Conjugate
    • 20
    • 4
    • 3
    • 3
    • 2
    • 2
    This P4HA1 antibody is un-conjugated
    Application
    • 29
    • 20
    • 8
    • 5
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human P4HA1. This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).
    Predicted Reactivity
    Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%) Mouse, Rat, Hamster, Opossum, Salmon, Stickleback, Pufferfish, Zebrafish (92%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide C-DFARKDEPDAFKE from an internal region of human P4HA1 (NP_000908.2, NP_001017962.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%), Mouse, Rat, Hamster, Opossum, Salmon, Stickleback, Pufferfish, Zebrafish (92%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product P4HA1 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))
    Alternative Name
    P4HA1 (P4HA1 Products)
    Synonyms
    P4HA antibody, AL022634 antibody, P4ha antibody, PHalphaI antibody, prolyl 4-hydroxylase subunit alpha-1 antibody, prolyl 4-hydroxylase subunit alpha 1 antibody, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide antibody, CpipJ_CPIJ000975 antibody, CpipJ_CPIJ017131 antibody, Tsp_03346 antibody, Tsp_03347 antibody, Tsp_15957 antibody, P4HA1 antibody, P4ha1 antibody
    Background
    Name/Gene ID: P4HA1

    Synonyms: P4HA1, 4-PH alpha-1, C-P4Halpha(I), p4HA, p4HA1
    Gene ID
    5033
    NCBI Accession
    NP_000908, NP_001017962
    UniProt
    P13674
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