P4HA1 antibody (AA 431-443)
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- Target See all P4HA1 Antibodies
- P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))
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Binding Specificity
- AA 431-443
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Reactivity
- Human, Dog, Pig, Cow, Horse, Rabbit, Monkey, Chicken, Bat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This P4HA1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human P4HA1. This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%) Mouse, Rat, Hamster, Opossum, Salmon, Stickleback, Pufferfish, Zebrafish (92%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-DFARKDEPDAFKE from an internal region of human P4HA1 (NP_000908.2, NP_001017962.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%), Mouse, Rat, Hamster, Opossum, Salmon, Stickleback, Pufferfish, Zebrafish (92%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product P4HA1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- P4HA1 (Prolyl 4-Hydroxylase, alpha Polypeptide I (P4HA1))
- Alternative Name
- P4HA1 (P4HA1 Products)
- Synonyms
- P4HA antibody, AL022634 antibody, P4ha antibody, PHalphaI antibody, prolyl 4-hydroxylase subunit alpha-1 antibody, prolyl 4-hydroxylase subunit alpha 1 antibody, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide antibody, CpipJ_CPIJ000975 antibody, CpipJ_CPIJ017131 antibody, Tsp_03346 antibody, Tsp_03347 antibody, Tsp_15957 antibody, P4HA1 antibody, P4ha1 antibody
- Background
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Name/Gene ID: P4HA1
Synonyms: P4HA1, 4-PH alpha-1, C-P4Halpha(I), p4HA, p4HA1 - Gene ID
- 5033
- NCBI Accession
- NP_000908, NP_001017962
- UniProt
- P13674
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