SPHK1 antibody (pSer225)

Catalog No. ABIN4620349

Product Details anti-SPHK1 Antibody

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Binding Specificity: pSer225

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects Sphingosine Kinase 1 (pSer225). Antibody shows some cross reactivity (~10 % by ELISA) with other phosphoserine containing proteins.

Purification: Affinity chromatography

Immunogen: A synthetic peptide incorporating the serine 225 phosphorylation site of mouse sphingosine kinase 1

Isotype: IgG

Application Details

Application Notes: Western Blot: (1-5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 0.08 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the product (in aliquots) at -20 °C. Can be shipped at 2 - 8 °C.

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Abstract: SPHK1 Products

Synonyms: CG1747 antibody, Dmel\\CG1747 antibody, SK1 antibody, Sphk1 antibody, SPHK1 antibody, F18E5.160 antibody, F18E5_160 antibody, sphingosine kinase 1 antibody, SPHK antibody, 1110006G24Rik antibody, Sk1 antibody, Spk1 antibody, Sphingosine kinase 1 antibody, sphingosine kinase 1 antibody, sphingosine kinase 1 L homeolog antibody, Sk1 antibody, SPHK1 antibody, sphk1.L antibody, Sphk1 antibody

Background: Activation of Sphingosine kinase (Sphk) increases the bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Phosphorylation of sphingosine by sphingosine kinase (Sphk) is the rate-limiting step in the cellular synthesis of S1P. A principle activation event is the phosphorylation of serine 225 of sphiongosine kinase 1. Inhibition of Sphk1 phosphorylation and hence, Sphk activity is an important target for the development of drugs for cancer, inflammation, cardioprotection as well as a many other indications.Synonyms: SPHK, SPK, SPK 1, SPK-1

Gene ID: 20698

NCBI Accession: NP_001165943

UniProt: Q8CI15

Pathways: VEGF Signaling