Western Blot: (1-5 μg/mL). Immunohistochemistry: (1-5 μg/mL). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
Phosphate buffered saline with 0.08 % Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
SPTLC1
(serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))
Sphingolipids play essential roles in various cellular events, including proliferation, differentiation, senescence, apoptosis, and inflammatory responses. Serine palmitoyltransferase (SPT) is the initial and rate-limiting enzyme in the de novo sphingolipid biosynthesis, and thus regulates the level of sphingolipids in cells. Immunohistochemical study revealed widespread distribution of the enzyme with most strong expression in brain and digestive tract. Two subunits, SPT1 and SPT2 at a stoichiometry of 1:1, are involved in the enzymatic activity of SPT.Synonyms: LCB 1, LCB1, Long chain base biosynthesis protein 1, SPT 1, SPT1, Serine palmitoyltransferase 1, Serine-palmitoyl-CoA transferase 1