CTDSP1 antibody (C-Term, N-Term)

Catalog No. ABIN360805

Product Details anti-CTDSP1 Antibody

Target: CTDSP1 (CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Small Phosphatase 1 (CTDSP1))

Binding Specificity: AA 1-250, C-Term, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTDSP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to CTDSP1-V250.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CTDSP1-V250 protein.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CTDSP1

Target: CTDSP1 (CTD (Carboxy-terminal Domain, RNA Polymerase II, Polypeptide A) Small Phosphatase 1 (CTDSP1))

Alternative Name: CTDSP1 (CTDSP1 Products)

Synonyms: CTDSP1 antibody, NIF3 antibody, NLI-IF antibody, NLIIF antibody, SCP1 antibody, fj83f12 antibody, im:7141428 antibody, wu:fj83f12 antibody, zgc:113169 antibody, GIP antibody, Nif3 antibody, CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 antibody, CTD small phosphatase 1 antibody, carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 antibody, CTDSP1 antibody, Ctdsp1 antibody, ctdsp1 antibody, HCAG_04820 antibody, CTRG_06220 antibody

Background: CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. Expression of CTDSP1 inhibited activated transcription from several promoter-reporter gene constructs, but expression of a mutant lacking phosphatase activity enhanced transcription. Neuronal gene transcription is repressed in nonneuronal cells by the repressor element-1 (RE1)-silencing transcription factor/neuron-restrictive silencer factor (REST/NRSF, 600571) complex. REST/NRSF recruits SCPs to neuronal genes that contain RE1 elements, leading to neuronal gene silencing in nonneuronal cells. Phosphatase-inactive forms of SCP interfere with REST/NRSF function and promote neuronal differentiation of P19 stem cells. Likewise, small interfering RNA directed to the single Drosophila SCP unmasks neuronal gene expression in S2 cells. Thus, SCP activity is an evolutionarily conserved transcriptional regulator that acts globally to silence neuronal genes.Synonyms: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1, NIF3, NLI-IF, NLI-interacting factor 3, NLIIF, SCP1, Small C-terminal domain phosphatase 1

Gene ID: 58190, 9606

UniProt: Q9GZU7