PARP1 antibody (AA 396-412)
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- Target See all PARP1 Antibodies
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
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Binding Specificity
- AA 396-412
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Reactivity
- Human, Dog, Horse, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PARP1 antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- KLH conjugated synthetic peptide comprising amino acids 396 - 412 [LTLGKLSRNKDEVKAMI] of the human ADP-ribosyltransferase (ADPRT) protein.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Horse (100%) Elephant (94%) Rat, Bovine, Rabbit, Opossum (88%) Mouse, Platypus (82%).
- Purification
- Protein G purified
- Immunogen
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Synthetic peptide from human PARP1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog, Horse (100%), Elephant (94%), Rat, Bovine, Rabbit, Opossum (88%), Mouse, Platypus (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product PARP1 Primary Antibody
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- Application Notes
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Approved: ELISA (1:1000), IHC, IHC-P (5 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))
- Alternative Name
- PARP1 / PARP (PARP1 Products)
- Background
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Name/Gene ID: PARP1
Synonyms: PARP1, ADPRT, ADP-ribosyltransferase NAD(+), Adp-ribosyltransferase, ADPRT1, ARTD1, ADPRT 1, Poly(ADP-ribose) synthetase, Poly(ADP-ribosyl)transferase, PARP, Poly [ADP-ribose] polymerase 1, PADPRT-1, PARP-1, Poly (ADP-ribose) polymerase 1, Poly(ADP-ribose) polymerase, Poly[ADP-ribose] synthase 1, PPOL - Gene ID
- 142
- UniProt
- P09874
- Pathways
- Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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